Sanger sequencing reaction mixtures included purified template DNA, a primer, DNA polymerase, and all four dNTPs. Each reaction also included a radiolabeled ddNTP version of just one of the four nucleotides. Only a small amount of each ddNTP was added, however, to ensure that a fraction of the total DNA fragments produced stopped at each occurrence of that base. As with previous methods, separating fragments via length and performing autoradiography allowed scientists to read the final sequence.
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Text: Naipanoi Lepapa, Ahmed Abdigadir, Julia Lindblom. Illustration: Erik Norman. Publicerad 2026-02-27 14:50
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我的原则就是我小时候被怎么对待,我不爽,那我尽量不去做(除了原则性问题)。对于学习来说,我也已经处于半随缘状态。不过新的一年,我希望自己可以做到: